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Fig. 7. Hob1 and Wsp1 cooperate with Nak1 to mediate cell growth and polarity. (A) SPU (wild type), RKO3B-4U (hob1 ${Delta}$ ), TYH1 (nmt1-nak1) and RK6B (hob1 ${Delta}$ nmt1-nak1) strains were grown on minimal medium with 100 µg ml^–1 thiamine and with or without 0.5 M KCl at the indicated temperature (30°C or 35°C) for 3-5 days. (B,C) Wild-type (SPU), wsp1 ${Delta}$ (WSP ${Delta}$ 3-12B) and hob1 ${Delta}$ (RKO3B-4U) cells expressing HA alone (control) or Nak1-HA from nmt1 promoter expression plasmids were grown on minimal medium for 3 days at 30°C and examined (B) or assayed for growth (C). (D) SPU (wild type), WSP ${Delta}$ 3-12B (wsp1 ${Delta}$ ), TYH1 (nmt1-nak1) and NW5D (wsp1 ${Delta}$ nmt1-nak1) strains were grown on minimal medium with 100 µg ml^–1 thiamine at 30°C for 3 days.

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