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Fig. 8. Hob1 and Wsp1 cooperate to mediate cell growth, division and polarity. (A) RNW2D (nmt1-wsp1 hob1
) cells were grown to mid-log phase in minimal medium in the absence and presence of 100 µg ml1 thiamaine and stained with calcofluor to observe cell septae. (B) Wild-type (RL143) cells co-expressing Wsp1-GFP and the myc epitope alone (control, left), myc-Hob1 (middle) or Hob11-281 (right) were grown in minimal medium and viewed for cell morphology and Wsp1-GFP localization.