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Fig. 4. Identification of the NoLS of mouse PARP-2. (Top) Schematic representation of the recombinant proteins, as described in Fig. 3. The substitution of 4-RRQR-7 to four alanines is indicated in red. Residues 15-50 and 33-46, involved in nuclear targeting, are underlined or boxed in green, respectively. (Bottom) Localization in living PARP-2–/– MEFs of the GFP-fused recombinant proteins (left panels) each labelled as in the scheme above. The corresponding DIC images are presented on the right. Bar, 10 µm.





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