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Fig. 3. The lack of cell-cell contacts in nhr-25(RNAi) L2 worms affects the fate of the seam-cell anterior daughters but not the ability of V5.p to produce a postdeirid neuron. Adherens junctions were stained with the anti-DLG-1 antibody. For simplicity, V cells are labeled with the names of their L1-stage ancestors. (A,B) In wild-type L2 worms, all seam cells remain in contact before and throughout their doubling division. V5.p produces an anterior neuroblast that is in the process of cell divisions, forming the future postdeirid (Pd). Contacts with descendants of V4.p and V6.p are shown by arrows. (C) In nhr-25(RNAi) animals, dividing seam cells remain round and isolated except for H1, whose contact with H0 is not interrupted because H1 had produced its hyp7 daughter towards the posterior. (D,E) The V5.pa daughters (brackets) divide and migrate dorsally in a postdeirid-like fashion regardless of the lack of contacts (arrowheads) with either V4.p (D) or V6.p (E) descendants in L2 nhr-25(RNAi) larvae. (F,G) Simultaneous propidium iodide and DLG-1 staining at the early L3 stage reveals the nuclei of the four postdeirid cells (Pd) and the normal adherens junctions (arrows) between V cells (F). The postdeirid structure differentiates normally in the absence of both anterior and posterior cell contacts of V5.p descendants (G, arrowheads) in rrf-3(pk1426); nhr-25(RNAi) worms. (H,I) Superfluous seam cells in nhr-25(RNAi) L2 worms express the scm::gfp marker (nuclear signal in H) and might form clusters (I). In all images, anterior is to the left and dorsal up. Bars, 10 µm; bar in A applies to all panels except C.

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