JCS01611 Supplemental Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Live-cell imaging of eGFP-Huntingtin exon1[Q138] dynamics. Time course images of mutant polyglutamine-expanded huntingtin exon 1 fragment aggregate dynamics at 2 frames per second over 30 seconds. 200 msecond exposure per frame taken at 37ºC.

• Movie 2 -

  Movie 2. Live-cell imaging of eGFP-ataxin-1[Q84] dynamics. Time course images of mutant polyglutamine expanded ataxin-1 nuclear inclusion dynamics at 2 frames per second over 30 seconds. 200 msecond exposure per frame taken at 37ºC.

• Movie 3 -

  Movie 3. Live-cell imaging of eGFP-ataxin-1[Q2] dynamics. Time course images of polyglutamine-removed human ataxin-1 nuclear inclusion dynamics at 2 frames per second over 30 seconds in human HeLa cells. 200 msecond exposure per frame taken at 37ºC.

• Movie 4 -

  Movie 4. Live-cell imaging of eGFP-ataxin-1[Q26] dynamics. Time course images of wild-type human ataxin-1 nuclear inclusion dynamics at 2 frames per second over 30 seconds in human HeLa cells. 200 msecond exposure per frame taken at 37ºC.

• Movie 5 -

  Movie 5. Live-cell imaging of eGFP-ataxin-1[Q84] dynamics. Time course images of polyglutamine-expanded mutant human ataxin-1 nuclear inclusion dynamics at 2 frames per second over 30 seconds in human HeLa cells. 200 msecond exposure per frame taken at 37ºC.

• Movie 6 -

  Movie 6. Live-cell imaging time course of a-amanatin treatment of eGFP-ataxin-1[Q84]-expressing cells. 25 mg/ml a-amanatin treatment time course over the last 2 hours of a 3 hour treatment at 37ºC showing dispersion of ataxin-1 nuclear inclusions. Time from addition of a-amanatin is indicated in lower left corner.