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Fig. 1. Ataxin-1 nuclear inclusions are dynamic and distinct from huntingtin exon1 polyglutamine aggregates. Three-dimensional (x,y,time) analysis of huntingtin and ataxin-1 nuclear inclusions by live-cell video microscopy. (A,B) Fluorescence images of eGFP-huntingtin exon1[Q138] and eGFP-ataxin-1[Q84] video sequences captured at 0.5-second intervals for 30 seconds at 37°C. (C,D,E,F) x,y,t voxel volume images of time sequences in orthogonal view; intensities thresholded to inclusions only in E and F. (G,H) Three-dimensional single representative inclusions of huntingtin and ataxin-1 with a dotted line as a straight reference. (I) A single ataxin-1 nuclear inclusion movement (see also Movie 1 in supplementary material). (J,K) Voxel volumes were rendered with an isometric surface projection to reveal a spiral structure indicating a spinning motion. Bars: (A-F) 10 µm, (G-K) 1 µm. J, K are presented as red (left eye) and cyan (right eye) stereo projections. Three dimensional x,y,t axis added for orientation reference.





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