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Fig. 5. Ataxin-1 nuclear inclusions co-localize with the TAP/NXF1 mRNA export factor. Laser confocal microscopy images of eGFP-ataxin-1 and mRFP-TAP/NXF1 (A-D), huntingtin exon-1[Q138] and mRFP-TAP/NXF1 (E-H) proteins. (I-L) Immunofluorescence with anti-TAP primary antibody and Alexa 594 secondary antibody on fixed NIH3T3 cell transfected with eGFP-ataxin-1[Q84] to detect endogenous TAP protein. (M-O) Cells transfected with mRFP-TAP/NXF1 alone, displaying no punctate localization (as in F and J). Bar: ~10 µm.





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