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Fig. 6. Ataxin-1 recruits TAP/NXF1 protein to nuclear inclusions as the result of cell heat-shock. Fluorescence micrographs of eGFP-ataxin-1[Q26] and mRFP-TAP/NXF1 in live cells after heat shock at 42°C for 5 minutes (A-C), followed by incubation for 1 hour at 37°C (I-J), with time course of TAP/NXF1 localization to ataxin-1 nuclear inclusions over 60 minutes shown in C-H. (K-M) Control of mRFP alone or expressed with eGFP-ataxin-1[Q26] after similar heat shock treatment. (N-P) Control of eGFP-PML protein co-expressed with TAP/NXF1-mRFP after heat shock as in panels A-J. Bar:
10 µm.