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Fig. 2. Oxidative stress-induced apoptosis in high glucose, high osmolarity and peroxynitrite. (A) Representative images of Hoechst 33258 nuclear staining show apoptotic cells that were identified based on their shrunken, irregular or fragmented nuclei. Quantification of these data is shown in Table 2. (B) A representative immunoblot, using cell lysates (50 µg), for cleaved PARP and ß-actin. Below: statistical analysis of quantification of cleaved PARP showing significant increases in apoptosis in cells treated with high glucose (HG), 3-O-methyl glucose (3mG) or peroxynitrite in comparison to cells cultured in normal glucose (NG). (*P<0.05 vs NG.)





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