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Fig. 3. Effect of RGTA on C2.7 cell differentiation. (A) C2.7 cells grown at high serum concentrations at day 4, without treatment (1) or in the presence of 0.5 µg/ml RGTA (2). C2.7 cells at 24 hours in differentiating medium (0.25% SVF + 0.25% SC) without treatment (3) or in the presence of 0.5 µg/ml RGTA (4). Arrows indicate myotubes in the cultures. (B) Subunits of creatine kinase (CK) and enzymatic activity at day 4 of C2.7 cell culture. Isoenzyme analysis was performed after agarose gel electrophoresis and the percentage of each subunit was calculated as described in Materials and Methods. CK activity is expressed as international units per dish. Bar, 100 µm.
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