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Fig. 4. Effect of RGTA on total sulfated GAG in C2.7 cellular extracts. Total sulfated GAG determinations were performed using the DMMB assay in proliferating and differentiating cells grown in the presence or absence of 0.5 µg/ml RGTA. The amounts of GAG were normalized by the amounts of DNA contained in each sample. Data are the mean±s.d. of duplicates or triplicates from four independent experiments. (A) Total GAG during proliferation. (B) Cell:medium ratio of total GAG during proliferation. (C) Total GAG during differentiation. (D) Cell:medium ratio of total GAG during differentiation. *P<0.05; **P<0.005; ***P<0.0005, when total GAG content or cell:medium ratios in RGTA-treated cells are compared with the respective controls.