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Fig. 1. Time course of BaCl₂-induced regeneration of tibialis anterior muscle (TA). TAs from C57BL/6 mice under control conditions or after 3, 5 and 7 days of BaCl₂-induced injury were dissected, counterstained and examined histologically (A) or analyzed for myogenin (Myo) levels (B). (A) The Hematoxylin and Eosin stained sections show that in the control most stained nuclei were located in the cell periphery. At 3 and 5 days post BaCl₂ injection (PI), fibers probably undergoing necrosis (arrows) and small mononucleated cells (<15 µm; arrowheads) were abundant in the muscle core. At 7 days PI, nearly 90% of the muscle section area was occupied by centrally nucleated myotubes. Scale bar: 60 µm. (B) Myogenin (Myo) was measured by western blot analysis in 100 µg protein aliquots of homogenates from control TA (C) and from TA at different days PI (3, 5, 7, 10 or 14). Differentiated C₂C₁₂ myoblasts were used as positive control.

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