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Fig. 5. Ice2p is found on the perinuclear and cortical ER. (A) Cells expressing a GFP fusion to Ice2p were grown at 30°C in minimal medium with the appropriate amino acids to early log phase and analysed by fluorescence microscopy. (B) Ice2p localizes with the ER marker Hmg1p-GFP. Ice2p was tagged at its C-terminus with 13x-Myc in cells expressing the ER marker Hmg1p-GFP (strain SFNY1283). The distribution of Ice2p was determined by indirect immunofluorescence using anti-myc monoclonal antibody. The first row shows Hmg1p-GFP. The anti-myc immunofluorescence is shown in the second row. The third row shows the merged image. The last row represents the differential-interference-contrast image.





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