QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. Overexpression of full-length or C-terminal cortactin enhances migration of mouse SCp2 mammary epithelial cells. (A) Domain structure of cortactin variants used in this study. FL, full-length cortactin; Nt, N-terminal (1-326 amino acids) cortactin; Ct, C-terminal (324-546 amino acids) cortactin; CtW525L, C-terminus of cortactin with W525L mutation; SH3, SH3 domain; SH3W525L, SH3 domain with W525L mutation. (B) Expression of cortactin variants in SCp2 cells. Western blot showing expression of the HA-FL, HA-Ct and HA-CtW525L cortactin (upper blot) or endogenous cortactin (lower blot). Constructs were expressed at or below the level of endogenous cortactin. Arrowheads denote HA-FL, HA-Ct and HA-CtW525L bands. V, control vector expression. (C) Migration of SCp2 cells in a representative wounding experiment is shown for control vector only infected (V) or cortactin variant infected (HA-FL, HA-Ct, HA-CTW525L) cells with images at 0 and 15 hours. (D) Quantification of wound closure data from three to seven experiments. Fold change in wound area was calculated as described in Materials and Methods. Error bars indicate s.e.m. and asterisks indicate statistically significant differences from change in area calculated for control cells (P<0.05).

Return to article