QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 3. Valproate induces the expression of ER-resident chaperones. (A) Relative levels of GRP78/BiP and GADD153/CHOP mRNA normalized to GAPDH mRNA as determined by northern blot analysis. HepG2 cells were cultured for the indicated times in the presence of 0.5 mM valproate. Total RNA was resolved by agarose gel electrophoresis, transferred to nylon membranes and subjected to blot hybridization with ³²P-radiolabeled cDNA probes encoding human GRP78/BiP and GADD153/CHOP. Relative mRNA levels were quantified using a Typhoon 9410 phosphoimaging system and normalized to GAPDH (loading control) RNA levels. (B) Immunoblot analysis of protein chaperone levels in HepG2 cells cultured for the indicated time in the presence of 0.5 mM valproate. Total protein lysates were resolved by SDS-PAGE, transferred to nitrocellulose membranes and immunostained with antibodies against GRP78/BiP, HSP47, calreticulin, PDI or HSP70 as indicated. As a loading control, an identical blot was immunostained with an antibody against ß-actin.

Return to article