Supplemental Figure 1 (Adobe PDF) -
Fig. S1. Alignment of the derived amino acid sequences of
genes Tbmbap1 and Tbmbap2
with those of the two Leishmania mexicana genes Lmxmbap (accession number Z46971) and Lmxmbap2
(accession number AJ303099) as well as of the gene for the L.
mexicana secreted acid phosphatase 1
precursor Lmxsap (accession number S54770) and a homologous sequence
from L. major (open reading frame
L2719.12, L. major sequencing
project). Residues conserved in all six sequences are printed in red, those
conserved in five sequences are printed in blue. Potential N-glycosylation
sites have been underlined. The known signal sequences of LmxMBAP and LmxSAP are boxed (Wiese et al., 1996). The putative transmembrane domains
of TbMBAP1, TbMBAP2, LmxMBAP,
and LmxMBAP2 as predicted from
Kyte-Doolittle hydrophobicity plots (GCG Wisconsin Sequence Analysis Package,
Madison, USA) are highlighted in grey. The two hallmark regions containing the
catalytically important histidine residues, RHGXRXP and YSAHDTT, are
highlighted in light blue. The residues used to raise anti-peptide antibodies
are shown in magenta.
