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Fig. 3. ROS accumulation in ${Delta}$ end3 and sla1 ${Delta}$ 118-511 cells. (A) ROS accumulation was assayed using H₂DCFDA by flow cytometry in wild-type, ${Delta}$ end3 and sla1 ${Delta}$ 118-511 cells grown to early stationary phase. (B) Graphical representation of the proportion of wild-type, ${Delta}$ end3 and sla1 ${Delta}$ 118-511 cells showing a high build up of ROS. (C) The relative sensitivity to H₂O₂ was calculated in wild-type, ${Delta}$ end3 and sla1 ${Delta}$ 118-511 using a halo-assay approach. (D) ROS accumulation was assessed using H₂DCFDA by flow cytometry in ${Delta}$ end3 and sla1 ${Delta}$ 118-511 cells, which were either rho⁰ strains or had been treated with 0.1 mg ml^–1 of the mitochondrial inhibitor antimycin A. (E) Exposure of the early apoptotic marker PS was measured using FITC/annexin-V in response to the addition of H₂O₂ at sublethal levels. A representative data set is shown.

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