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Fig. 7. Sla1p localization to the cortex in ${Delta}$ end3 cells is enhanced by RSP5 overexpression. (A) Wild-type, ${Delta}$ end3 and ${Delta}$ end3[RSP5] cells expressing an integrated Sla1p-GFP were examined by fluorescence microscopy. In wild-type cells, Sla1p-GFP appeared as punctate spots that appear largely within the buds of dividing cells. (B) In ${Delta}$ end3 cells, Sla1p appeared largely diffuse and cytoplasmic, and in a few punctate cortical structures. (C) In ${Delta}$ end3[RSP5] cells, the cytoplasmic staining was reduced and an increase in punctate cortical staining was observed in both mother and bud. Bar, 10 µm.

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