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Fig. 9. Reduced actin dynamics of ${Delta}$ end3 cells are increased by PDE2 overexpression. (A) F-Actin was examined in overnight cultures of ${Delta}$ end3 cells carrying either the YEp13 or YEp13 plasmid containing PDE2. (B) LatA-sensitivity halo assays were carried out on cells from wild-type cultures containing YEp13, wild-type cultures containing YEp13[PDE2], ${Delta}$ end3 cultures containing YEp13 and ${Delta}$ end3 cultures containing YEp13[PDE2]. (C) Glycogen accumulation was assessed in cells from wild-type cultures containing YEp13, ${Delta}$ end3 cultures containing YEp13 and ${Delta}$ end3 cultures containing YEp13[PDE2] cultures. (D) Heat-shock sensitivity was assessed in wild-type cultures containing YEp13, wild-type cultures containing YEp13[PDE2], ${Delta}$ end3 cultures containing YEp13 and ${Delta}$ end3 cultures containing YEp13[PDE2] cells. Bar, 10 µm.

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