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Fig. 10. Effect of cholesterol depletion on FRAP kinetics for Kv2.1, Kv1.4 and Kv1.3. HEK cells were cholesterol depleted by incubation with 2% 2-hydroxypropyl-ß-cyclodextrin (CD) and FRAP measured as described in Materials and Methods. Average recovery after photobleach for YFP-Kv2.1HA (A), YFP-Kv1.4myc (B) and YFP-Kv1.3 (C). The dashed line in each plot represents the FRAP under control conditions for each channel, and solid lines are exponential fits to the average data following cholesterol depletion. Fit parameters – Kv2.1 (n=8): {tau}1=51.0 seconds, A1=0.12; {tau}2=2889.1 seconds, A2=0.54; Mf=0.32±0.03. Kv1.4 (n=9): {tau}=195.7 seconds, A=0.39; Mf=0.58±0.07. Kv1.3 (n=18): {tau}1=39.2 seconds, A1=0.34; {tau}2=535.7 seconds, A2=0.42; Mf=0.89±0.06.





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