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Fig. 3. YFP-Kv2.1HA and YFP-Kv1.4myc exhibit a differential polarization in HEK cells. Confocal images of YFP fluorescence in live cells were taken of the bottom surface of expressing cells (top panels in both A and B). Three-dimensional confocal stacks were used to generate X-Z images of each cell, which were two-dimensional deconvolved using Zeiss LSM510 v3.2 software (bottom panel, A and B). Kv2.1 clearly has a preference for the basal surface, whereas Kv1.4 is evenly distributed. Bars, 10 µm.