spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. YFP-Kv1.4myc and YFP-Kv1.3 are freely mobile in the plasma membrane. (A) Images from FRAP times series in a YFP-Kv1.4myc-expressing HEK cell. The region highlighted in the white box was bleached and recovery in that region followed by scanning every 15 seconds for a total of 80 scans (see Materials and Methods). (B) Average recovery after photobleach for YFP-Kv1.4myc. Fluorescence at each time point was normalized to prebleach intensity and averaged for all cells (n=16). The solid line through the data is a single exponential fit to the average data (see Materials and Methods). Fit parameters: {tau}=133.0 seconds, A=0.52; Mf=0.78±0.07. (C) Average recovery following photobleach for YFP-Kv1.3 (n=31). Curve and fit was generated as for YFP-Kv1.4 with the solid line representing a single exponential fit. Fit parameters: {tau}=117.6 seconds, A=0.56; Mf=0.78±0.04. Bar, 10 µm.





Right arrow Return to article