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Fig. 7. Use of photoactivatable-GFP to observe diffusion of Kv1.4. Photoactivation and imaging was performed as described in Fig. 5. No fluorescence is apparent before photoactivation of PA-GFP-Kv1.4myc; following illumination by 405 nm light, there is bright green fluorescence in the ROI. Unlike PA-GFP-Kv2.1, ~1 minute after activation, there is GFP signal apparent throughout the cell membrane, consistent with rapid diffusion of Kv1.4 throughout the plasmalemma. Approximately 13 minutes after photoactivation, PA-GFP-Kv1.4 is evenly distributed over the entire cell membrane. Bar, 10 µm.





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