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Fig. 2. Expression of the ZO-17-444 fusion protein in transfected ROS cells. (A) ROS/ZO-1dn cells were harvested and analyzed by immunoblotting with the Xpress tag antibody. A 54 kDa immunoreactive band is seen in the transfected cells. The position of molecular mass markers is given in kDa. (B) Localization of the ZO-17-444 fusion protein in ROS/ZO-1dn cells. ROS and ROS/ZO-1dn cells (clone E) were fixed, permeabilized, and stained with Xpress epitope tag antibody. Scale bar: 25 µm.





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