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Fig. 3. Expression of the ZO-1^7-444 fusion protein disrupts the Cx43-ZO-1 interaction. (A) Cx43 associates with the ZO-1^7-444 fusion protein in transfected cells. ROS/ZO-1dn cells were solubilized in lysis buffer and lysates were fractionated on a cobalt chelate affinity resin. Bound material was analyzed by immunoblotting with Cx43 antiserum. Cx43 was detected in precipitates from high expressor ROS/ZO-1dn clones E, J, and K, but not in ROS or low expressor ROS/ZO-1dn clone A cells. (B) ROS/ZO-1dn cells were solubilized, immunoprecipitated with Cx43 antiserum, run on SDS-PAGE, and immunoblotted with an anti-ZO-1 monoclonal antibody. ZO-1 co-precipitated with Cx43 in ROS and in the low expressor ROS/ZO-1dn clone A cells, but not in high expressor ROS ZO-1dn clones E, J, or K. The position of molecular mass markers is given in kDa.

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