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Fig. 6. Expression of the ZO-17-444 fusion protein reduces appositional membrane staining for Cx43 in transfected ROS cells. (A) Cells were fixed, permeabilized and simultaneously stained with the Sigma polyclonal antibody directed against Cx43 and a monoclonal antibody directed against ZO-1. (B) Expression of ZO-1 in transfected ROS cells. ROS cells and transfected ROS cells were analyzed by immunoblotting with the ZO-1 monoclonal antibody. Scale bar: 25 µm.





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