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Fig. 7. Expression of the ZO-17-444 fusion protein alters the mobility of Cx43 on sucrose flotation gradients. ROS and ROS/ZO-1dn cells were harvested in 1% Triton X-100, equilibrated in 40% sucrose, overlaid with a discontinuous 5-30% sucrose gradient, and centrifuged. Gradient fractions were collected, separated by SDS-PAGE and Cx43, ZO-1 and caveolin-1 were identified by immunoblotting. Fraction 1 was derived from the bottom of the sucrose gradient (40% sucrose), while fraction 9 was derived from the top of the sucrose gradient (5% sucrose).





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