JCS02352 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Supplementary Fig. S1. Transgenic mouse genotyping. (A) Southern blot analysis of genomic DNA from wild-type (wt) and transgenic (tg) founder mice using the specific hGH-probe (Fig. 1) for transgene detection. The probe hybridized to a 4 kb fragment in all transgenic animals. Junction fragments are labeled with *. (B) PCR analysis of genomic DNA from animals from a line 60 litter using specific primers for detection of the transgene (Fig. 1). The positive control (+) is plasmid DNA. There was a positive correlation between the presence of the transgene in genomic DNA and coat morphology of the mice.

• Supplemental Figure 2 -

  Supplementary Fig. S2. Hair length in ECR3E transgenic mice. (A) Hairs were plucked from three ECR3E transgenic (tg) and three wild-type (wt) littermates. From each mouse an average of 12 hairs of the monotrich type and 30 hairs of the awl or zigzag types were measured. Values represent means + standard deviation of the total length from three mice. Each type of hair from 15-day-old transgenic mice was 30% shorter compared with hair from wild-type littermates. (B) On day 15 after birth, back hairs were plucked from ECR3E-Myc and ECR3-1-2-Myc mice and the length of the zigzag hairs measured. Similar results were obtained with monotrich hairs (data not shown).