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Fig. 1. LTBP-1, ECR3E-Myc, ECR3E-1-2-Myc and transgene constructs. (A) LTBP-1 structure and its binding to LAP and TGF-ß through the CR3 region. Ovals represent CR domains, rectangles EGF-like domains; LAP is in black, and mature TGF-ß in orange. (B) The amino acid sequence of the CR3 region of the LTBP-1 (ECR3E-Myc) and the mutations introduced to convert ECR3E-Myc into CR3 that resembles that of LTBP-2 (ECR3E-1-2), which does not bind TGF-ß. The amino acid changes are boxed and the deleted amino acids are labeled with arrows. (C) Transgene products. The two CR3 regions (oval) surrounded by the EGF-like repeats (rectangles) tagged with Myc tag (long rectangle) that were used in making the transgene constructs represented in D. (D) K14 transgene constructs. The keratin 14 (K14) promoter was used to drive the expression of ECR3E-Myc constructs into the basal layer of the epidermis. The human growth hormone 3' untranslated region and poly A (hGH poly A) were added for stabilization of the transcript. Primers used for PCR are represented by arrows and the fragment of the hGH used for Southern blotting indicated by the bold lines.





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