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Fig. 4. Expression of the transgenes in vivo. (A) The Myc-tagged transgenes were detected using anti-Myc antibody in sections of back skin from 9-day-old mice. Both ECR3E-Myc and ECR3E-1-2-Myc transgenic animals showed labeling in the basal layer of the epidermis and the outer root sheet (ORS) of the hair follicle. Control (WT) littermates did not show significant labeling of these regions. (B) Expression of the TGF-ß1 and LTBP-1 visualized using RNA extracted from isolated epidermis or cultured keratinocytes followed by RT-PCR. (C) Wild-type and transgenic (ECR3E-Myc) mice at day 11.





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