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Fig. 6. Detection of phosphorylated Smad2/3 (pSmad2/3) in the skin of wild-type and transgenic animals during the first hair cycle. (A) Immunocytochemistry using pSmad2/3 antibody and Alexafluor visualization of activated Smad2/3 on sections from 9-day-old mice. The positive pSmad2/3 signal stained red. The DAPI-stained nuclei, which were blue, were artificially colored green. Therefore, cells with pSmad2/3 in the nucleus appeared yellow-orange because of the red green overlap. Green arrows indicate pSmad-negative nuclear staining, yellow arrows indicate pSmad-positive nuclear staining. (B) The number of pSmad-positive cells in hair follicles is represented as a percentage of the total number of cells in at least eight fields from each sample of skin from animals 0 to 26 days after birth. The follicles in the skin of ECR3E-Myc animals have higher levels of positive cells compared with the follicles of the wild-type and ECR3E-1-2-Myc.





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