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Fig. 3. Assembly of the CD95 DISC is independent of CD95 capping. (A) Jurkat and H9 cells were assessed for the presence of caspase-8 in the CD95 DISC by immunoprecipitation of CD95 after stimulation with {alpha}CD95 for the indicated time. (B) Jurkat and H9 cells were pretreated as in Fig. 2A and were then subjected to immunoprecipitation of CD95 after stimulation with {alpha}CD95 for 10 minutes. Cell lysates and IP samples were analyzed for the presence of caspase-8. The treatments that abolished CD95 capping did not inhibit recruitment of caspase-8 and assembly of the CD95 DISC. Representative immunoblots from two separate experiments are shown.





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