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Fig. 4. Actin colocalizes with CD95 capping, giving the cells a polarized phenotype. (A) Jurkat cells were treated with 
CD95 for 10 minutes at 37°C to stimulate the cells. Fixed cells were then labeled with Alexa Fluor 488 phalloidin to detect F-actin. Maximum projections from confocal z-sections were generated and representative cells from at least three separate experiments are shown. F-actin rapidly reorganizes to one pole of the cell upon CD95 activation. (B) Jurkat and (C) H9 cells were treated and labeled for CD95 (green) as in Fig. 1. The fixed and permeabilized cells were then further labeled with Alexa Fluor 546 phalloidin (red). Finally, single confocal z-sections were acquired. Overlay pictures of representative cells from at least three separate experiments are shown. CD95 colocalizes with F-actin only when CD95 capping is allowed (yellow).
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