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Fig. 5. CD95 stimulation induces rapid aggregation of lipid rafts. (A) Lipid rafts (GM1) in Jurkat cells were labeled with Alexa Fluor 555 CTxB and the cells were then treated with 
CD95 for 10 minutes at 37°C. The fluorescence images show that CD95 stimulation rapidly induced aggregation of the lipid rafts to one pole of the cells. (B) Jurkat and (C) H9 cells treated as in Fig. 3 were labeled with Alexa Fluor 555 CTxB (red) and CD95 (green). Cells were kept on ice (0') or transferred to 37°C for 10 minutes (10') to induce capping. CD95 colocalizes with lipid rafts only when CD95 capping is allowed (yellow). Single confocal z-sections were acquired and overlay images of representative cells from three separate experiments are shown.
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