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Fig. 4. Translocation of complexin II after stimulation. Complexin II in RBL-2H3 was visualized with anti-complexin II antibody or anti-myc antibody, using FITC-conjugated anti-mouse IgG, as in Fig. 1C. (A) Distribution of complexin II before antigen stimulation. The left image shows the distribution of complexin II in wild-type cells using anti-complexin II antibody. The right image shows the distribution of myc-tagged complexin II in cells transfected with myc-tagged complexin II using anti-myc antibody. (B) Distribution of complexin II at 5 minutes after antigen stimulation. Complexin II was translocated to the plasma membrane. Left and right images show the distribution of complexin II and myc-tagged complexin II, respectively. (C) Distribution of complexin II at 5 minutes after antigen stimulation in the absence of extracellular Ca²⁺. Complexin II was translocated to the plasma membrane. (D) Timecourse of antigen-induced degranulation in the absence of extracellular Ca²⁺. Values were obtained from four independent preparations of wild-type cells [mean±s.e.m. (n=4)]. (E) Distribution of complexin II at 5 minutes after thapsigargin (50 nmol/l) stimulation in the absence of extracellular Ca²⁺. Complexin II was translocated to the plasma membrane. (F) Timecourse of thapsigargin-induced degranulation in the absence of extracellular Ca²⁺. Values are obtained from four independent preparations of wild-type cells [mean±s.e.m. (n=4)].

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