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Fig. 6. Effects of antisense oligonucleotides directed against the RYR subtypes on Ca²⁺ oscillations induced by rapamycin. (A) Typical Ca²⁺ oscillations induced by 10 µM rapamycin on permeabilized cells. (B) Compiled data showing the amplitude of Ca²⁺ responses evoked by 10 µM rapamycin in non-injected cells (white bar) and cells injected with 10 µM asRYR antisense oligonucleotides (black bars). (C) Percentage of oscillating cells in the same conditions as in B. Data are means ± s.e.m. with the number of tested cells indicated in parentheses. Myocytes were loaded with 2 µM Fluo4-AM and used 3 days after oligonucleotide injection (stars indicate P<0.05).

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