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Fig. 3. Fragmentation of the cohesin core occurs at diplotene in the absence of the SYCP3. Late-diplotene oocytes were stained with antisera against SYCP1 (B,D, green; F,H,J,L, red) and CREST (white) in combination with anti-SMC1ß (red), anti-REC8 (green) and anti-SMC3 (green) antibodies. (A,B,E,F,I,J) Wild-type oocytes. (C,D,G,H,K,L) Sycp3–/– oocytes; arrows indicate some of the centromeres. Notice the persistence of cohesin signal at all centromeres (see supplementary material, Fig. S2 for more detail). Bars, 10 µm.





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