|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 3. Dispersal of TGN and Golgi elements in cells depleted of tGolgin-1. HeLa cells transduced twice with siRNA-1 were fixed 1 day later. They were then either analysed by IFM using primary antibodies to TGN46 and to the indicated proteins, followed by the appropriate fluorochrome-conjugated secondary antibodies (A-K), or embedded in epon and analysed by electron microscopy. (C-K) tGolgin-1-depleted cells were identified based on the dispersed pattern of TGN46 staining. (K) A colorized overlay of the images shown in I,J to emphasize the polarity of the Golgi and TGN elements; the boxed region is magnified 2.5 times in the top right corner. (L-Q) Characteristic electron microcopy fields containing Golgi membranes in cells transduced with control siRNA (L) or siRNA-1 (M-Q). Notice the reduced size of the stacks in cells transduced with siRNA-1. Bar in Q (applies to L-Q), 0.2 µm.
|
