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Fig. 4. Association of Golgi elements with microtubules in cells depleted of tGolgin-1. (A-F) HeLa cells transduced twice with siRNA-1 (A-C) or with dynamitin-p50 (D-F) were fixed 1-2 days later and analysed by deconvolution IFM using primary antibodies to TGN46 and -tubulin. (C,F) Overlays of the two labels. The boxed regions are magnified 2.5 times in the bottom or top right corner to emphasize the alignment of Golgi elements along microtubules. (G-R) HeLa cells were transfected with GalT-EGFP alone and left untreated (Ctl.; G-I) or treated with nocodazole (Noc.; J-L), or cotransfected with GalT-EGFP and dynamitin-p50 (Dyn-p50; M-O) or with GalT-EGFP and siRNA-1 (P-R). Cells were fixed, labelled with anti--tubulin and RRX-conjugated secondary antibodies, and analysed by deconvolution fluorescence microscopy. Colorized overlays of the two labels are shown on the right. (S-X) Untransduced cells treated for 2 hours with nocodazole (Noc.; V-X) or cells transfected with dynamitin-p50 (S-U) were fixed and analysed by deconvolution IFM using primary antibodies to TGN46 and giantin. (U,X) Overlays of the two labels; the boxed regions are magnified 2.5 times in the top right corners.

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