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Fig. 2. Western blot analysis of IP3R1 in PM and MA muscle fiber cultures. (A) Whole cell protein extracts (200 µg for IP3R1 and 10 µg for ${alpha}$ -actin) from innervated (+SC) and non-innervated (–SC) PM and medial adductor MA muscle fibers were electrophoresed and immunoblotted. IP3R1 and ${alpha}$ -actin were detected with a rabbit polyclonal antibody and mouse polyclonal antibody, respectively, followed by HRP-conjugated secondary antibodies and chemiluminescent substrates. (B) The abundance of IP3R1 in innervated (+SC) and non-innervated (–SC) PM and MA muscle fibers was quantified by using ${alpha}$ -actin as a loading control for normalization Innervation significantly (P<0.05) increased IP3R1 abundance in both PM and MA muscle fibers, and IP3R1 abundance was significantly (P<0.05) increased in innervated and non-innervated PM muscle fibers relative to that in MA muscle fibers. Bars represent mean±s.e.m., n=4.

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