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Fig. 4. Effect of IP3R1 inhibition on protein kinase C (PKC) activity. PM muscle fibers were cultured with and without innervation by spinal cord (SC) explants. Medium in some of the cultures was supplemented with 100 µM 2-APB to inhibit IP3R1 activity. After 7 days in culture, PKC activities were determined as described in Materials and Methods. Both innervation and inhibition of IP3R1 activity significantly (P<0.05) reduced PKC activity. Bars represent mean (±s.e.m.) PKC activity (n=4).





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