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Fig. 5. Effect of innervation and IP3R1 inhibition on slow MyHC2 promoter activity. PM muscle fibers were cultured with and without spinal cord (SC) explants either in control medium or medium supplemented with 2-APB. Slow MyHC2 promoter activity was significantly (P<0.05) increased in innervated PM muscle fibers cultured in medium containing 2-APB. Innervation or IP3R1 inhibition alone did not significantly increase slow MyHC2 promoter activity. Bars represent mean (±s.e.m.) fold increase in activity compared to levels in the control (n=6).





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