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Fig. 6. Effect of innervation and IP3R1 inhibition on MEF2 and NFAT-dependent transcription. PM muscle fibers were transfected with MEF2 and NFAT promoter sensor constructs. Some of the cultures received spinal cord (SC) explants for innervation and half of the cultures were incubated in control medium or medium supplemented with 100 µM 2-APB to inhibit IP3R1 activity. Innervation significantly (P<0.05) increased both MEF2 and NFAT-dependent transcription in PM muscle fibers incubated in control medium. IP3R1 inhibition by 2-APB in non-innervated PM muscle fibers did not significantly affect MEF2-mediated transcription, but did significantly (P<0.05) reduce NFAT-dependent transcription. Inhibition of IP3R1 activity by 2-APB in innervated PM muscle fibers did not significantly affect MEF2-mediated transcription compared to innervated muscle fibers in control medium. NFAT-dependent transcription was significantly (P<0.05) reduced in innervated PM muscle fibers cultured in medium with 2-APB compared to control medium. Bars represent means±s.e.m., n=6.





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