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Fig. 6. Scratch-wound experiments. The center areas of 3-week confluent monolayers of ARL cells grown on coverslips were removed by scratching with a pipette tip, and the incubation was continued in fresh medium for 30 hours. Cells were stained with McAb 351 (A), or double-stained with McAb 351 (B1) and an anti-vimentin antibody (B2). (C) After scratching, ARL cells were further incubated in the presence or absence of aphidicolin (20 µg/ml) for 30 hours. Cells were stained with McAb 351. In all panels, right-hand areas correspond to the marginal regions of cleared areas made by scratching. Bar, 50 µm.





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