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Fig. 1. Domains and constructs investigated in this study. (A) Stathmin family proteins: except for stathmin, each protein possesses, in addition to its stathmin-like domain, an N-terminal extension including the conserved domain A (domain A, dark grey; domains A' and A'', symbols). (B) Sequence alignment of domains A from SCG10, SCLIP and RB3/RB3'/RB3'': conserved residues are highlighted in grey, and the two palmitoylated cysteines in black. The delineation of the three regions n (grey), m (black) and c (white) was driven by the sequence conservation pattern. (C) A first series of GFP fusion proteins was constructed with the domain A from SCG10 or with various combinations of its derived subdomains (n+m, m+c, m, n); an additional pair of constructs encoding the palmitoylated N-terminal domain of GAP-43 (hatched) fused or not to region n (lower two constructs) was also engineered to further investigate the mechanism of action of this region.
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