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Fig. 3. Subcellular distribution of GFP fusion proteins containing domain A of SCG10 or derived subdomains in HeLa cells. A-GFP, a_nm-GFP, a_m-GFP, a_mc-GFP were expressed in HeLa cells. After 24 hours, the cells were fixed and labelled with the CTR433 monoclonal Golgi marker, and examined by fluorescence confocal microscopy. GFP fluorescence and CTR433 immunofluorescence images for each fusion protein, with the corresponding overlay image in the right-hand column (GFP, green; CTR433, red). A-GFP (A) is localized to the Golgi complex, as shown by its colocalization with the CTR433 labelling, and on punctate structures, which may correspond to vesicles. a_m-GFP (a_m) and a_mc-GFP (a_mc) are present at the Golgi complex but also at the plasma membrane, whereas a_nm-GFP (a_nm) is restricted to the Golgi and punctate structures, like the full-length domain A construct. All images are projections from confocal stacks. Bar, 10 µm.

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