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Fig. 3. Subcellular distribution of TACE-eGFP changes with the coexpression of active ERK2. (A) HeLa cells were transfected with either 0.3 µg pWT.TACE-GFP or pT735A.TACE-eGFP, with or without 0.1 µg of pCA.MEK-1.HA and/or pWT.ERK2-HA. DNA amounts were kept equivalent using pcDNA3.1+/Zeo. Cells were fixed in methanol and stained for constitutively active MEK-1 ({alpha}-MEK-1), WT.ERK-HA ({alpha}-HA), or active phosphor-ERK2 ({alpha}-pERK2 in B), before being visualised using laser-scanning confocal microscopy. TACE-eGFP, CA.MEK-1 and/or ERK2 are green and red respectively, whereas the nuclei are blue. The arrowheads highlight a change in the subcellular location of TACE-eGFP protein. Bar, 10 µm.





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