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Fig. 2. The relationship between maximally shortened muscle length (normalized by the in situ length) and the isotonic load (expressed as a percentage of maximal isometric force). Solid circles are data obtained at plateaus of isotonic contractions under various loads and fitted with a linear line (solid). The dotted lines represent a reference length-force relationship from skeletal muscle [reproduced from Gordon et al. (Gordon et al., 1966)] with the sarcomere length of 2.0 µm superimposed on the in situ length of the trachealis. The open circles and filled squares are data obtained after quick stretches of magnitudes of 10% and 30% Lin situ, respectively, that show isometric forces at the stretched lengths and final shortened lengths (arrow) in isotonic contractions under a constant load of 30% Fmax.





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