JCS02366 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. The ER network of Ciona intestinalis oocyte. Confocal sections (1 µm) from the surface to the equator. The animal pole is facing the upper right corner. The ER is labeled with DiIC16(3).

• Movie 2 -

  Movie 2. Mitochondria in Ciona intestinalis oocyte. Confocal sections (2 µm) from the surface to the equator. Individual mitochondria are seen as bright green dots. Larger vesicles in light green correspond to yolk platelets. Labeling with DiOC2(3).

• Movie 3 -

  Movie 3. Transition zone from animal to vegetal hemisphere showing the distribution of  mitochondria (green) and ER (red) under the surface of a Ciona oocyte. Confocal sections.

• Movie 4 -

  Movie 4. Time-lapse confocal sequence of a Phallusia zygote (equatorial sections) from fertilization to mitosis showing the first major phase of reorganization (vegetally directed contraction) and the second major phase of reorganization (posterior translocation). Images were acquired every 5 minutes. The animal pole is in the upper right corner. Mitochondria are in green, ER in red. The bright red droplet corresponds to the site of injection for DiIC16(3).

• Movie 5 -

  Movie 5. (1575 K) Time lapse confocal sequence of a Phallusia zygote (equatorial sections) undergoing meiotic contractions (see Roegiers et al., 1999) pronuclear migration and the second major phase of reorganization. Images were acquired every minute. Mitochondria are labeled with DiOC2(3) (green) and ER with DiIC16(3) (red). The bright red droplet corresponds to the site of injection for DiIC16(3).

• Movie 6 -

  Movie 6. Time-lapse confocal sequence of a Phallusia embryo undergoing unequal cleavages in the posterior pole from the 8 to the 32 cell stages. ER is labeled with DiIC16(3) (red) and mitochondria with DiOC2(3) (green). Arrow shows the cER-rich CAB. Images were acquired every 2 minutes.