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Fig. 6. Hsc70-auxilin-ATP mixture does not dissociate adaptors or transferrin from clathrin-coated pits. GFP-clathrin cells were uncoated with Hsc70, auxilin, and ATP for 10 min, at which time they were fixed. Cells were immunostained for clathrin (a,b), AP2 (c,d) and epsin (e,f) both before (a,c,e) and after uncoating (b,d,f). The GFP-clathrin cells were preloaded with transferrin, permeabilized, and imaged before (g) and after uncoating (h). Bars, 10 µm.





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